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BioNB 4300 - Experimental Molecular Neurbiology Laboratory

Syllabus - Spring 2015

Time/Location:  TBD, Lab, Tuesday all day
Instructor:  David Deitcher dld14@cornell.edu (OH by appointment)
Course website:  http://courses.cit.cornell.edu/bionb4300/

Lab Report Guidelines

Lab or Section Date

Topic

1.

January 27

Quiz 1
Lab 1 - Enhancer traps and reporter genes

Larval Dissection Movie

2.

January 29

Section - Discussion of lab 1 and handout for lab 2

3.

February 3

Quiz 2
Lab 2 - NMDA receptor cDNA isolation Part I

4.

February 5

Section - Discussion of lab 2 and handout for lab 3

5.

February 10

Quiz 3
Lab 3 - NMDA receptor cDNA isolation Part II

Bluescript KS map: http://www.fermentas.com/en/support/technical-reference/phage-plasmid-dna/pbluescriptII

1 kb ladder.jpg, 100 bp ladder.jpg ,Lab3Gels_Bluescript.jpg, Lab3Gels_PCRproducts.jpg

6.

February 12

Report 1 Due
Section - Discussion of lab 3 and handout for lab 4

7.

February 17

Quiz 4
Lab 4 - NMDA receptor cDNA isolation Part II

Lab4 Gel Bottom Row.jpg, Lab4 Gel Top Row.jpg

8.

February 19

Section - Discussion of lab 4 and handout for lab 5

9.

February 24

Quiz 5
Lab 5 - NMDA receptor cDNA isolation Part IV

NMDA_review, NMDAR3, NR1_cloning

10.

February 26

Section - Discussion of lab 5 and handout for lab 6

11.

March 3

Quiz 6
Report 2 Due
Lab 6 - Western blotting of mutant head extracts Part I

Westernblot part I supplemental

12.

March 5

Section - Discussion of lab 6 and handout for lab 7

13.

March 10

Quiz 7
Lab 7 - Western blotting of mutant head extracts Part II

WesternBlotResults.jpg, Vilinsky_et_al.pdf, Rao_et_al.pdf

14.

March 12

Section - Discussion of lab 7 and handout for lab 8

15.

March 17

Quiz 8
Report 3 Due
Lab 8 - Immunoprecipitation of proteins Part I

 

16.

March 19

Section - Discussion of lab 8 and handout for lab 9

17.

March 24

Quiz 9
Lab 9 - Immunoprecipitation of proteins Part II

 

18.

March 26

Section - Discussion of lab 9 and handout for lab 10

19.

April 7

Quiz 10
Report 4 Due
Lab 10 - Molecular Identification of wavy Part I

20.

April 9

Section - Discussion of lab 10 and handout for lab 11

21.

April 14

Lab 11 - Molecular Identification of wavy Part II

22.

April 16

Section - Discussion of lab 11 and handout for lab 12

23.

April 21

Quiz 11

Report 5 Due

Lab 12 – Bang Sensitive Flies Part  I

24.

April 23

Section - Discussion of lab 12 and handout for lab 13

25.

April 28

Lab 13 – Bang Sensitive Flies Part  II

26.

 

27.

April 30

 

May 5

May 11

Section Discussion of lab 13 and handout for lab 14

 

 Final Data Analysis of Class Data for Bang Sensitive Lab

Report 6 Due

 

 

Grading

Quizzes 15%
Quizzes will be given at the beginning of the days indicated.  The quizzes are designed to make sure you have carefully read the laboratory exercises to be performed that day before you come to lab.  They will be short (lasting only 10 minutes).  The lowest quiz grade may be dropped.
Laboratory Report 1 (Enhancer trap Lab) 10%
Laboratory Report 2 (NMDA Receptor Lab) 15%
Laboratory Report 3 (Western Blotting Lab) 15%
Laboratory Report 4 (Immunoprecipitation Lab) 15%
Laboratory Report 5 (Molecular Identification of wazy Lab) 15%

Laboratory Report 6 (Bang Sensitive Flies) 15%